Hello Kim, Unfortunately your question is beyond the scope of this list as we do not provide advice regarding scientific direction but focus on browser usage issues.
Best regards, Pauline Fujita, Ph.D. UCSC Genome Bioinformatics Group http://genome.ucsc.edu On 09/27/11 20:53, Kim Spradling wrote: > Hello- > > I am planning to sequence the transcriptome of 700 human lymphocyte > samples. In order to calculate the number of samples to run per lane > to achieve 30X coverage, I need to determine the size of the human > transcriptome. Is it correct to use the number of block bases > (71,851,762) listed in the RefSeq table for the Feb 2009 (GRCh37/hg19) > assembly? > > Thanks so much for your help! > Kim > > ____________________________________ > Kimberly D. Spradling, Ph.D. > Postdoctoral Scientist, Department of Genetics > Texas Biomedical Research Institute > 7620 NW Loop 410 > San Antonio, TX 78254 > Phone: 210-258-9624 > > _______________________________________________ > Genome maillist - [email protected] > https://lists.soe.ucsc.edu/mailman/listinfo/genome _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
