On Fri, May 02, 2014 at 01:47:50PM +0200, [email protected] wrote:
> my question is the following. I have to calculate the roughness with its
> error bar from images with 3 nm height islands on silicon oxide.

This is impossible.  More precisely, it is impossible without any a
priori information telling you what is the topography and what is the
error/uncertainty of topography.

If you calibrated the instrument
(http://gwyddion.net/documentation/user-guide-en/caldata.html) and
applied the calibration to the data then you would know a priori the
random and systematic errors for each data point.  Gwyddion would
perform corrections and provide uncertainties of the statistical
characteristics automatically in the Statistical Quantities tool (and a
few other places).

Otherwise you may be able to roughly estimate uncertainties of some
statistical quantities only if you have a model to which the surface
must conform.  This allows you to state that all deviations are
measurement errors.  For instance, if you can say a part of the surface
is so flat that all observed variations from plane are measurement
errors, you can then estimate these errors.

Since roughness itself is a random deviation from some mean shape, the
situation is even more complex here.  Generally, you need at least a
statistical model of the roughness.  You can try to just estimate type A
uncertainties by repeated measurements, and this may be what you are
doing, however
- This is likely a small part of the total uncertainty; the main issue
  is not the variation between individual measurements but systematic
  errors due to noise, tip convolution effects, limited area effects, ...
- The Row/Column Statistics tool does not do this anyway.

So, what Row/Column Statistics does?  It calculates the selected
quantity for all rows or columns and displays the average value and
inter-row or inter-column variation.  This is not an error estimate!

There is probably only a single quantity whose average tells something
about the entire surface: the mean.  The average of means is the mean
for the entire surface.  All other quantities would have to be combined
in a more complex manner to obtain something describing the entire
surface or they are purely 1D quantities.  So the average does not
correspond directly to a 2D quantity and the variation is not the error
of some 2D quantity.

I hope this, at least, clears up things.

Regards,

Yeti


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