I"ve seen underfixed tissue stain with an ugly background everywhere for kappa/lambda (so much that you can't tell what's real staining and what isn't). I think it depends on your retrieval. Especially if you're digesting for retrieval, and it wasn't properly fixed, I can imagine all kinds of staining issues.
On Wed, Nov 12, 2008 at 10:00 AM, Gudrun Lang <[EMAIL PROTECTED]> wrote: > Thank you for your answers. An additional question: Can someone confirm, > that underfixed tissue shows false positive kappa and lambdastaining? > > Bye > Gudrun > > -----Ursprüngliche Nachricht----- > Von: [EMAIL PROTECTED] > [mailto:[EMAIL PROTECTED] Im Auftrag von Gudrun > Lang > Gesendet: Mittwoch, 12. November 2008 16:45 > An: histonet@lists.utsouthwestern.edu > Betreff: [Histonet] experiences with kappa/lambda > > Hi listmembers, > > Please tell me about your experiences with the IHC-demonstration of kappa > and lambda on FFPE-sections. > > We have some troubles about inconsistend staining and therefore false > results about the monoclonality of tumors. > > Bye > > Gudrun > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet