Yep, Extensive NBF fixation tends to rinse out the extra-cellular immunoglobulins and so decreases its contribution to the background staining.
Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Gudrun Lang Sent: Thursday, 13 November 2008 5:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] kappa/lambda and.. Thank you for your answers. An additional question: Can someone confirm, that underfixed tissue shows false positive kappa and lambdastaining? Bye Gudrun -----Ursprüngliche Nachricht----- Von: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Im Auftrag von Gudrun Lang Gesendet: Mittwoch, 12. November 2008 16:45 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] experiences with kappa/lambda Hi listmembers, Please tell me about your experiences with the IHC-demonstration of kappa and lambda on FFPE-sections. We have some troubles about inconsistend staining and therefore false results about the monoclonality of tumors. Bye Gudrun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
