What kind of control slides are you using? If the marking is on the same side as the tissue , it could prevent your solution from getting to all your tissue. Rena Fail
-------------- Original message from "Angela Bitting" <akbitt...@geisinger.edu>: -------------- > Here's one for the Ventana BenchmarkXT users out there: > > We mount the patient tissue on the same slide with our control tissue. The > control stains beautifully, but there is absolutely no staining in the > patient > tissue. This will happen to one or two out of 30 slides on a run. It also > happened on two different instruments. Over the last week this has occurred 6 > times. Different protocols, different machines. > I tend to think its a deparaffinization issue. Can one side of a pad heat and > the other end not reach temp? We haven't noticed that the slides moved during > staining or were not seated properly on the pad. > Help me figure this one out folks!! > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > > > > > IMPORTANT WARNING: The information in this message (and the documents > attached > to it, if any) is confidential and may be legally privileged. It is intended > solely for the addressee. Access to this message by anyone else is > unauthorized. > If you are not the intended recipient, any disclosure, copying, distribution > or > any action taken, or omitted to be taken, in reliance on it is prohibited and > may be unlawful. If you have received this message in error, please delete > all > electronic copies of this message (and the documents attached to it, if any), > destroy any hard copies you may have created and notify me immediately by > replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet