I think your explanation of the problem is probably the most likely Karen.
________________________________ From: histonet-boun...@lists.utsouthwestern.edu on behalf of Bauer, Karen L. Sent: Mon 2/2/2009 3:02 PM To: renaf...@bellsouth.net; Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] perplexed We also had this happen, and the red control box marking was on the underside of the slide. Seems that when the slides are packaged in the box, the front part of the slide is pressed with the red markings on the back of the slide in front of it. This creates an invisible barrier on the non-marked side and can trap the solution from spreading to the whole slide, which prevents the patient tissue from being stained. We got rid of our red control box slides and are just using plain charged slides. That problem has NEVER happened again. There are slides with an etched box for the control tissue (etching is on the back I believe) if you want to try those, but we decided to just stick with what works the best. Good Luck, Karen Karen L. Bauer HT(ASCP) Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.ka...@mayo.edu -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of renaf...@bellsouth.net Sent: Monday, February 02, 2009 2:49 PM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] perplexed What kind of control slides are you using? If the marking is on the same side as the tissue , it could prevent your solution from getting to all your tissue. Rena Fail -------------- Original message from "Angela Bitting" <akbitt...@geisinger.edu>: -------------- > Here's one for the Ventana BenchmarkXT users out there: > > We mount the patient tissue on the same slide with our control tissue. > The control stains beautifully, but there is absolutely no staining in > the patient tissue. This will happen to one or two out of 30 slides on > a run. It also happened on two different instruments. Over the last > week this has occurred 6 times. Different protocols, different machines. > I tend to think its a deparaffinization issue. Can one side of a pad > heat and the other end not reach temp? We haven't noticed that the > slides moved during staining or were not seated properly on the pad. > Help me figure this one out folks!! > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email However many electrons > were severly inconvienienced! > > > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. > It is intended solely for the addressee. Access to this message by anyone else is unauthorized. > If you are not the intended recipient, any disclosure, copying, > distribution or any action taken, or omitted to be taken, in reliance > on it is prohibited and may be unlawful. If you have received this > message in error, please delete all electronic copies of this message > (and the documents attached to it, if any), destroy any hard copies > you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet