Angela, I've had similar issues with the Ventana Nexus special stainer - specifically on the retic and trichrome. We have always mounted the patient tissue on the same slide as the control tissue. About 6 months ago, we started have inconsistent staining. Ventana investigated thoroughly, but we didn't come up with any answers. So now, we have to stain the patient tissue separately from the control tissue - which doubles our cost since we have two slides instead of one. Please let me know if you figure anything out.
Laurie Colbert -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Monday, February 02, 2009 12:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] perplexed Here's one for the Ventana BenchmarkXT users out there: We mount the patient tissue on the same slide with our control tissue. The control stains beautifully, but there is absolutely no staining in the patient tissue. This will happen to one or two out of 30 slides on a run. It also happened on two different instruments. Over the last week this has occurred 6 times. Different protocols, different machines. I tend to think its a deparaffinization issue. Can one side of a pad heat and the other end not reach temp? We haven't noticed that the slides moved during staining or were not seated properly on the pad. Help me figure this one out folks!! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet