Hello histonet this is a call for any advice. I am a purely lab-taught histotech based in the UK and have been studying part-time to enable suitable accreditation and for my final year project am intending to use the large back catalogue of blocks in the forensic lab I work in to study cardiac changes associated with obesity (this is of special interest in the West of Scotland where I work).
My intention is to select cases where obesity is judged (by pathologist) to be a primary or secondary factor in cause of death and where other factors (i.e. hypertension) were not indicated in life and compare with an age-matched cohort to asses the correlation between obesity and cardiac changes such as degree of interstitial fibrosis. Initially I intend to take a single case with well developed obesity-linked cardiac changes and do a range of stains (H&E, MSB, EVG, PTAH, a range of trichromes, PSR) to determine which stains to use in the whole study. I would be very grateful to hear from anyone who has undertaken similar work with advice on range of stains/techniques or tips on papers I should read or with any other advice. Also any advice on immuno that I could undertake. Thank you Finlay _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet