Hi Everyone!! I am perfusing CO2 euthanized 3 weeks old mice with PBS only. I am nicking the upper right atrium of heart to collect the gushed out blood and then perfusing through ventricle using a 21G butterfly needle and peristaltic pump. Sometimes I see the lungs swelling up and fluid comes out of mouth. Occasionally, I see the liver fade to light pink. Most of the time the paws become white. But the brain and spinal cord (my tissues of interest) are always white, and seem to have been perfused. I was wondering how to improve this to get more consistent good perfusions, and what signs should I look for to indicate good perfusion?
Thanks much, Dzung NIAID _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
