Aazath, Several possibles: It can come down to the ripening time of the haematoxylin. Hx with rapid ripening (more oxidiser, sodium iodite or (heaven forbid!) mercury oxide, added) will tend to stain strongly initially but tend to over-oxidise giving weak or brown nuclear staining.
Heating the solution and adding the oxidiser will cause the same thing. Less oxidiser will give you a solution with a longer self life but weaker staining. If the pH is too low, staining will be weaker, but more specific. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Aazath Raj Sent: Wednesday, 2 September 2009 1:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E preparation Dear All, We are preparing harris heamtoxylin for H&E in our laboratory. some time we get intense nuclear staining some time light weak.Does anybody know how to control a consistency in the strength . Aazath Technical Officer Apollo Hospitals India _________________________________________________________________ News, sports, entertainment and fine living...learn the ropes on MSN India http://in.msn.com_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet