Ripening in the sun (we do get some in the UK) used to give Haematoxylin that was stable; if you forgot to make any up then you hastely added oxidising agent to a new batch or 'jumped started' a batch that was as yet still immature. That batch then never seemed to last as long as you got muddy staining. They looked quite pretty, lines of 5 litres flasks with their contents ripening in the sun with cotton wool balls sticking out of the top, next to the cigarette ashtrays. How things have changed..............
Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Aazath Raj Sent: 01 September 2009 16:49 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E preparation Dear All, We are preparing harris heamtoxylin for H&E in our laboratory. some time we get intense nuclear staining some time light weak.Does anybody know how to control a consistency in the strength . Aazath Technical Officer Apollo Hospitals India _________________________________________________________________ News, sports, entertainment and fine living...learn the ropes on MSN India http://in.msn.com_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet