Ah, the good 'ol days! Remember adding the oxidizinng agent (mecuric oxide) too soon when it was still bubbling and having a volcano of hematoxylin?!!! LOLj Was remembering tho', that every day, before using, we would "top off" (add...oh, about 30ml) of fresh to the filtered used batch. This really kept the quality good. Worked for 30 yrs until we started purchasing it. Gone are the morning coffee and donuts sitting next to our microtomes too!! Sigh................ Ya, ya....I know!!!
>>> "Kemlo Rogerson" <kemlo.roger...@waht.swest.nhs.uk> 09/02/09 3:53 AM >>> Ripening in the sun (we do get some in the UK) used to give Haematoxylin that was stable; if you forgot to make any up then you hastely added oxidising agent to a new batch or 'jumped started' a batch that was as yet still immature. That batch then never seemed to last as long as you got muddy staining. They looked quite pretty, lines of 5 litres flasks with their contents ripening in the sun with cotton wool balls sticking out of the top, next to the cigarette ashtrays. How things have changed.............. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Aazath Raj Sent: 01 September 2009 16:49 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E preparation Dear All, We are preparing harris heamtoxylin for H&E in our laboratory. some time we get intense nuclear staining some time light weak.Does anybody know how to control a consistency in the strength . Aazath Technical Officer Apollo Hospitals India _________________________________________________________________ News, sports, entertainment and fine living...learn the ropes on MSN India http://in.msn.com_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet