Dalton's Chrome-osmium fix was popular for awhile in the 1950's. You
will need to look in an older methods book. The combo of dichromate and
osmium will not make your safety officer happy. There are many ways to
stain lipids with osmium, alone or mixed with something else. Is this
for EM or LM?
Geoff
Liz Chlipala wrote:
I'm trying to find a procedure for staining fat in tissues with osmium
and I think potassium dichromate, does anyone out there have this
protocol available. I have found it before on the web but for some
reason I can't find it this time.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcaul...@umdnj.edu
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