OSMIUM TETROXIDE FOR FAT (Paraffin SECTIONS) Solutions 2.5% Potassium Dichromate Solution Stock 1% Osmium Tetroxide Solution Stock
Potassium Dichromate-Osmium Tetroxide Solution Working Potassium Dichromate Stock 50 ml Osmium Tetroxide stock 50 ml Staining Procedure 1. Place formalin fixed specimen, no thicker than 4mm, in working potassium dichromate-osmium tetroxide solution for up to 16 hours. 2. Wash specimen in running water for 2 hours. 3. Process tissue, embed in paraffin and cut at 4 microns. 4. Stain in routine H&E staining setup, than mount. Results: Fat - black Background - same as H&E stain. Hope this helps. If you have any questions please let me know. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, December 02, 2009 3:13 PM To: histo...@pathology.swmed.edu Subject: [Histonet] procedure for osmium and potassium chromate? I'm trying to find a procedure for staining fat in tissues with osmium and I think potassium dichromate, does anyone out there have this protocol available. I have found it before on the web but for some reason I can't find it this time. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet