Dear all,
I am sorry if this is a bit of a basic question. I would like to observe localization of fluorescent microparticles embedded in mouse skin. I plan to do this simply by cryosectioning skin, mounting the tissue without fixation, and observing under the fluorescence microscope (the particles are added before killing the mouse). However, I would also like to counterstain the tissue without losing the fluorescence of my sample (so I can see similar features as I might using H&E). Can anyone suggest a good way to do this? I would be very grateful for any advice. Many thanks Nick Evans _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
