Dear Nick Evans, First: Say who and where you are, and who is in charge of your experiments with mice. Second: Tell your boss to buy two or three histotechnology textbooks (about $50 each) and allow himself and you and all your colleagues 30 mins paid daily reading/lunch time. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Nicholas David Evans <ndev...@stanford.edu> Date: Friday, January 8, 2010 20:18 Subject: [Histonet] Counterstain for fluorescent tissue To: histonet@lists.utsouthwestern.edu
> Dear all, > > > > I am sorry if this is a bit of a basic question. I would like to > observelocalization of fluorescent microparticles embedded in > mouse skin. I plan > to do this simply by cryosectioning skin, mounting the tissue without > fixation, and observing under the fluorescence microscope (the > particlesare added before killing the mouse). > > > > However, I would also like to counterstain the tissue without > losing the > fluorescence of my sample (so I can see similar features as I > might using > H&E). Can anyone suggest a good way to do this? I would be very > gratefulfor any advice. > > > > Many thanks > > Nick Evans > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
