This can also be a fixation issue. The edge fixes faster than the center....
Colleen Forster
V. Neubert wrote:
Is it just unevenly stained? Or are your sections unevenly thick!?
Hi,
I have a question for the generous input. When I do the IHC or IF, it
seems very common that the intensity of the edge area of the tissue is
always stronger than the central tissue part. Is it possible to
eliminate this and make the staining evenly distributed around the whole
tissue section?
Your kind help is greatly appreciated,
Thanks in advance,
Best,
Karen
Karen Cai
Research Scientist
Prosci Incorporated
(858) 513-2638 x 204
(858) 513-2692 Fax
<http://www.prosci-inc.com> www.prosci-inc.com
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