Here is the reference: Effect of Slide Drying at 80Deg C on Immunohistochemistry A.F. Henwood J Histotechnology, VOl. 28, no. 1, March 2005
If you are an NSH member you can email the NSH office and ask for a pdf reprint. The author compares heating the slides at 80C for seven hours to one hour at 65C. Some antigens were adversely affected, some were not. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pat Laurie Sent: Friday, February 05, 2010 12:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide baking before IHC Histonet, I have heard rather anecdotally that if you bake slides at high temps (75 degrees to 80 degrees) before IHC for a long period of time (several hours to days), you may affect antigenicity for some antibodies. Has there been any study done about this? Also, what if it is a high temp (around 75 degrees C) for just 20 minutes? Or a low temperature for a long period of time? Thanks in advance for your input. -- Patrick Laurie HT(ASCP)QIHC plau...@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
