I concur with Tim, this paper is a good reference for slide drying temps, as with everything else there is no rule that works for every antigen, I play it safe and try very hard to air dry draining until there is no water left on or under the section, and then I bake at temps not above 60dc for most things, but when it comes to bone and cartilage, I drain and air dry them and then lay them flat on heat plate at 45 dc overnight when possible.
Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, February 05, 2010 10:24 AM To: Pat Laurie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide baking before IHC Here is the reference: Effect of Slide Drying at 80Deg C on Immunohistochemistry A.F. Henwood J Histotechnology, VOl. 28, no. 1, March 2005 If you are an NSH member you can email the NSH office and ask for a pdf reprint. The author compares heating the slides at 80C for seven hours to one hour at 65C. Some antigens were adversely affected, some were not. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pat Laurie Sent: Friday, February 05, 2010 12:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide baking before IHC Histonet, I have heard rather anecdotally that if you bake slides at high temps (75 degrees to 80 degrees) before IHC for a long period of time (several hours to days), you may affect antigenicity for some antibodies. Has there been any study done about this? Also, what if it is a high temp (around 75 degrees C) for just 20 minutes? Or a low temperature for a long period of time? Thanks in advance for your input. -- Patrick Laurie HT(ASCP)QIHC plau...@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet