Here is a procedure that I found in the archives. http://www.histosearch.com/histonet/Jul01A/Siriusredcollagenprocedur.html
And here is another... Sirius Red Stain Picro-sirius red Sirius Red F3B (CI 35780) Aldrich 36,554-8 synonym Direct Red 80 0.5gm Saturated aqueous Picric Acid 500ml [add a small amount of solid picric acid to ensure saturation: 1.2~1.3% (W/V)] Expiration date minimum 3 years Acidified Water Acetic Acid (glacial) 5 ml Water (DI or tap) 1 liter Ø Fix frozen sections 30 minutes in Neutral Buffered Formalin(For paraffin sections deparaffinize and bring slides to water) Ø Wash sections in 3 changes of water Ø Drain sections well and place into Picro-Sirius solution 2 min Ø Wash sections in 2 changes of acidified water(5min) Ø Dehydrate in 3 changes of 100% ethanol (5min) Ø Clear in xylene and mount in permanent mounting media (5min).b I found the powder here.... http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=43665|FLUKA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC<http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=43665%7CFLUKA&N5=SEARCH_CONCAT_PNO%7CBRAND_KEY&F=SPEC> On Wed, Feb 24, 2010 at 12:22 PM, Nails, Felton <[email protected]>wrote: > Is there a good Sirius Red procedure used to stain collagen > And where can I find the powder > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------ > CONFIDENTIALITY NOTICE: > The information in this e-mail may be confidential and/or > privileged. If you are not the intended recipient or an > authorized representative of the intended recipient, you > are hereby notified that any review, dissemination, or > copying of this e-mail and its attachments, if any, or > the information contained herein is prohibited. If you > have received this e-mail in error, please immediately > notify the sender by return e-mail and delete this e-mail > from your computer system. Thank you. > ============================================================ > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
