You'll find brief instructions for the picro-sirius red method for collagen in nearly every histological techniques book published since about 1970. A Google search for "sirius red vendor" brings up 484 hits, though not all are for vendors. It's important to get the correct dye (C.I. 35780, Direct red 80). For a thorough discussion of how to use the method intelligently, see L. Junqueira et al 1979. Histochem. J. 11: 447-455.
John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: "Nails, Felton" <[email protected]> Date: Wednesday, February 24, 2010 12:23 Subject: [Histonet] Sirius Red To: "[email protected]" <[email protected]> > Is there a good Sirius Red procedure used to stain collagen > And where can I find the powder > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------ > CONFIDENTIALITY NOTICE: > The information in this e-mail may be confidential and/or > privileged. If you are not the intended recipient or an > authorized representative of the intended recipient, you > are hereby notified that any review, dissemination, or > copying of this e-mail and its attachments, if any, or > the information contained herein is prohibited. If you > have received this e-mail in error, please immediately > notify the sender by return e-mail and delete this e-mail > from your computer system. Thank you. > ============================================================ > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
