A few drops of saturated aqueous lithium carbonate added to the 70% alcohol step will do the job nicely.

Geoff

cscam...@uci.edu wrote:
Hi Histonet,

After placing slides in Bouin's for 1 hour at 56 degrees, I am finding it
very difficult to remove the yellow coloring. Rinses with water are not
doing the trick. Does anyone have some advice on how to bring the slides
back to a clear color so that I may proceed with the Masson Trichrome
procedure?

Thanks!
-Colin


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**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 mcaul...@umdnj.edu
**********************************************



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