This is great FYI, Jan. Our lab works a lot with pig tissues, too. And we,
too, have found in our lab that a number of human Abs appear to work on pig
tissues. Still, I can definitely appreciate the difficulties in finding a
pig-specific antibody, dealing the unknowns of cross-reactivity and degree
of homology.
Jan, I will certainly keep you in mind when we are seaching for a
porcine-reacting antibody! Thanks for sharing that.
Regards,
Merced
--On Thursday, March 18, 2010 7:48 AM -0500 Jan Shivers <shive...@umn.edu>
wrote:
I work on all species, porcine being one of them. I've never done a %
survey of the number of Abs made against human epitopes that also work on
porcine tissue, but I can give you generalizations.
All of the rabbit polyclonal antibodies that I've tried have
cross-reacted with porcine tissue. The polyclonals that I use the most
are the Abs against hormones, neuroendocrine markers, vasculature,
astrocytes, etc.
Most of the mouse monoclonal antibodies that I've tried have
cross-reacted with porcine tissue. These would be the intermediate
filaments, melanoma antigens, cell proliferating antigens, neuroendocrine
markers, etc.
You may have more difficulty with leukocyte markers (CD markers). They
seem to have less homology with human markers than the above items, and
not all cross-react, in my experience. You only learn this by trial and
error, unfortunately, since data about species cross-reactivity is rare.
I have 2 polyclonal and 2 monoclonal CD markers that cross-react with
porcine. That is not to say that there aren't more, but that's all that
I currently have worked up on porcine tissue.
I have not tried Abs like ER, PR, CEA, TTF, etc.
I've done species cross-reactivity studies with every antibody I have in
stock (>80), so if you send me a list of the antibodies you're interested
in (directly to me), I can let you know which ones cross-react with
porcine tissue, and which vendor works for me.
Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN 55108
612-624-7297
shive...@umn.edu
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----- Original Message ----- From: "RICKY MATHIS"
<cmmath...@bellsouth.net>
To: "histonet" <histonet@lists.utsouthwestern.edu>
Sent: Wednesday, March 17, 2010 8:15 PM
Subject: [Histonet] Anti-human abs work on porcine tissue
I work with some porcine tissues and it is sometimes difficult to find
antibodies specific to pig. A doctor I work with asked some one he knew
in Europe about using antibodies that are listed as working in human
being used on porcine tissue. This person stated that there would be
about a 10% chance that the anti-human antibodies would work on porcine
tissues. The antibody companies mostly give the same "we did not try it
on pig tissue" response. I understand that it is likely expensive to
continue to test antibodies on a wide variety of animal tissues, so that
is fine. But what do you guys think about the 10% chance? I would have
said more than that.
Thank you in advance for your time,
Cathy
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)
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