Me three, please. :-)
I just discovered we have this same antibody in our lab but my boss was
unsuccessful with it (he had tried 95oC for 45 min, pH 6.0).
Thanks!
Merced
--On Tuesday, April 13, 2010 2:24 PM -0500 shive...@umn.edu wrote:
Me, too, please.
Jan Shivers
UMN Vet Diag Lab
On Apr 12 2010, Liz Wilson wrote:
Hi Patsy,
I would love to try the method! Thanks -Liz
Liz Wilson
Bethyl Labs
________________________________________________________________________
____ Message: 7
Date: Sat, 10 Apr 2010 10:33:02 -0600
From: "Patsy Ruegg" <pru...@ihctech.net>
Subject: RE: SPAM-LOW: [Histonet] Re: Cd31 in pigs
To: "'Liz Wilson'" <lwil...@bethyl.com>,
<histonet@lists.utsouthwestern.edu>
Message-ID: <59adba33d53b44778565f88cbd7fa...@prueggihctechlt>
Content-Type: text/plain; charset="us-ascii"
A few years ago I spent a lot of effort on using Serotec's mouse
anti-porcine CD31 for ffpe porcine tissue and I did get some positive
results with unusual pretreatment methods, as I recall I had to use a
waterbath for hier set at 70dc for 3 hours in hph buffer. I will look up
the method at work on Monday and send it to you if you contact me again
to remind me.
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)
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However, many electrons were severely inconvenienced.
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