Biocare antibody. Using the diluent that they recommend. High pH retrieval for 20 minutes. And the Dako kit with the mouse linker.
Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean [jtay...@meriter.com] Sent: Thursday, August 19, 2010 10:44 AM To: 'histonet@lists.utsouthwestern.edu'; 'ih...@googlegroups.com' Subject: [Histonet] PAX-5 - B-cell specific activator protein (BSAP) Hi Everyone, I'm trying to find out what company and clone most labs are using for PAX-5 (BSAP). Thanks! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, August 18, 2010 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 81, Issue 23 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: CD34 positive control (McMahon, Loralee A) 2. Ventana Renaissance Containers (ricky hachy) 3. CD34 Control (Silverman, Jeffrey) ---------------------------------------------------------------------- Message: 1 Date: Wed, 18 Aug 2010 12:17:39 -0400 From: "McMahon, Loralee A" <loralee_mcma...@urmc.rochester.edu> Subject: RE: [Histonet] CD34 positive control To: Joel Reichensperger <jreichensper...@siumed.edu>, Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <c27aa2a01cef31469813089e226f582e02d5a7c...@urmcms7.urmc-sh.rochester.edu> Content-Type: text/plain; charset="us-ascii" Just about any tissue should work. It stains for Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts We use a sausage style control here with about 30 different types of tissue. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joel Reichensperger [jreichensper...@siumed.edu] Sent: Wednesday, August 18, 2010 12:05 PM To: Histonet Subject: [Histonet] CD34 positive control I have a doctor who wants to stain some tissue with cd34. I need to know if anyone can recommend a good positive control tissue for this antibody. The staining will be done in paraffin embedded sections. Thanks in advance. Joel -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute jreichensper...@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 18 Aug 2010 16:28:39 +0000 From: ricky hachy <elc...@hotmail.com> Subject: [Histonet] Ventana Renaissance Containers To: Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <snt114-w54ab5f08e7e0cfc97193edcf...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Hello , I need 4 good plastic containers, where you fill with xilene,alcool....... for the VENTANA RENAISSANCE . Anyone could sell me ? Regards Ricky ------------------------------ Message: 3 Date: Wed, 18 Aug 2010 12:30:58 -0400 From: "Silverman, Jeffrey" <jsilver...@nshs.edu> Subject: [Histonet] CD34 Control To: "'jreichensper...@siumed.edu'" <jreichensper...@siumed.edu> Cc: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <83f4d81747a7094dafe3ae87151ecb941ce4144...@sykechxvs01.nslijhs.net> Content-Type: text/plain; charset="us-ascii" Joel, A section of skin will be a fine CD34 control. All collagenous connective tissue, including the dermis, are rich in CD34+ dendritic interstitial fibroblasts as well as CD34 positive endothelium in the resident vessels. Actually, in my lab we use a section of fallopian tube from tubal ligation, they are loaded with the fibroblasts and vessels. Jeff Silverman ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 81, Issue 23 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet