Yes, I meant distilled water.. but now there is no problem.. I just checked my cut sections that were mistakenly cryoprotected in sucrose/DW instead of in sucrose/PBS and there is not a significant degree of cell lysis.. the dorsal root ganglion neurons are mostly intact and not lysed.. I felt happy yest and decided to be more careful the next time I do cryoprotection of my tissues..
Anyway thanks Sarah for your comment.. Byee.. Rags, Postdoctoral Research Fellow, Verge Lab, Department of Anatomy and Cell Biology, Room No: 5800, Saskatoon City Hospital, 701, Queen Street, Saskatoon, Saskatchewan S7K 0M7, Canada. Phone (Lab): +1 306-655-8714 (Facsimile): +1 306-655-8709 --- On Wed, 25/8/10, sgoe...@xbiotech.com <sgoe...@xbiotech.com> wrote: From: sgoe...@xbiotech.com <sgoe...@xbiotech.com> Subject: RE: [Histonet] Re: Cryoprotection To: "Mejia,Maria" <maria.me...@ucsf.edu> Cc: "vaigunda ragavendran" <rags_...@yahoo.co.in>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Date: Wednesday, 25 August, 2010, 12:53 AM I assumed distilled water? Sarah Goebel, B.A., HT (ASCP)Histotechnician XBiotech USA Inc.8201 East Riverside Dr. Bldg 4 Suite 100Austin, Texas 78744(512)386-5107 -------- Original Message -------- Subject: RE: [Histonet] Re: Cryoprotection From: "Mejia, Maria" <maria.me...@ucsf.edu> Date: Tue, August 24, 2010 10:31 am To: vaigunda ragavendran <rags_...@yahoo.co.in>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Can you be more specific & tell us what DW means & please give us the protocol for the sucrose including the amount of DW used. This way we can help you. Regards Maria Mejia Histology Supervisor Department of Neurosurgery UCSF San Francisco, CA ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of vaigunda ragavendran [rags_...@yahoo.co.in] Sent: Friday, August 20, 2010 1:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Cryoprotection Hii, If instead of sucrose in PBS, we use sucrose in DW by mistake, what might happen to fixed tissue sections during cryoprotection.. Please lemme know.. Rags, Postdoctoral Research Fellow, Verge Lab, Department of Anatomy and Cell Biology, Room No: 5800, Saskatoon City Hospital, 701, Queen Street, Saskatoon, Saskatchewan S7K 0M7, Canada. Phone (Lab): +1 306-655-8714 (Facsimile): +1 306-655-8709 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet