I assumed distilled water? Sarah Goebel, B.A., HT (ASCP)
Histotechnician XBiotech USA Inc. 8201 E= ast Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: RE: [Histonet] Re: Cryoprotection From: "Mejia, Maria" <[1]Maria.Meji= a...@ucsf.edu> Date: Tue, August 24, 2010 10:31 am To: vaigunda ragavendran <[2]rags_j= e...@yahoo.co.in>, "[3]histo...@lists.utsout= hwestern.edu" <[4] histonet@lists.utsouthwestern.edu> Can you be more specific & tell us what DW means & please give us t he protocol for the sucrose including the amount of DW used. This way we can help you. Regards Maria Mejia Histology Supervisor Department of Neurosurgery UCSF San Francisco, CA ________________________________________ From: [5]histonet= -boun...@lists.utsouthwestern.edu [[6]histonet-boun...@lists.utsouthwestern.edu] On = Behalf Of vaigunda ragavendran [[7]rag= s_...@yahoo.co.in] Sent: Friday, August 20, 2010 1:19 PM To: [8]histo...@lists.uts= outhwestern.edu Subject: [Histonet] Re: Cryoprotection Hii, If instead of sucrose in PBS, we use sucrose in DW by mistake, what might h= appen to fixed tissue sections during cryoprotection.. Please lemme know.. Rags, Postdoctoral Research Fellow, Verge Lab, Department of Anatomy and Cell Biology, Room No: 5800, Saskatoon City Hospital, 701, Queen Street, Saskatoon, Saskatchewan S7K 0M7, Canada. Phone (Lab): +1 306-655-8714 (Facsimile): +1 306-655-8709 _______________________________________________ Histonet mailing list [9]histo...@lists.utsouth= western.edu [10]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [11]histo...@lists.utsouth= western.edu [12]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:maria.me...@ucsf.edu"; 2. 3D"mailto:rags_...@yahoo.co.in"; 3. 3D"mailto:histonet@lists.utsouthwestern.edu"; 4. 3D"mailto:histonet@lists.utsouthwestern.edu"; 5. 3D"mailto:histonet-boun...@lists.utsouthwestern.edu"; 6. 3D"mailto:histonet-boun...@l 7. 3D"mailto:rags_...@yahoo.co.in"; 8. 3D"mailto:histonet@lists.utsouthwestern.edu"; 9. 3D"mailto:Histonet@lists.utsouthwestern.edu"; 10. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"; 11. 3D"mailto:Histonet@lists.utsouthwestern.edu"; 12. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"; _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
