The longer the tissue sits in paraformaldehyde the more the proteins in the
tissues will become cross-linked due to multiple methylene bridge
formations and could require very rigorous antigen retrieval or may even be
beyond retrieval; the tissues could become brittle as well due to
over-fixation. Just my 2 cents.
Regards,
Merced
--On Wednesday, September 22, 2010 3:39 PM -0600 Salim Yalcin Inan
<syi...@ucalgary.ca> wrote:
Hello,
I have a question about post-fixation. As far as I know, for most of the
immunohistochemistry studies in mouse/rat brain, post-fixation period is
between 2 hours to 24 hours in 4% paraformaldehyde at +4 Celsius followed
by cryoprotection with 20-30% sucrose solution. Are there any
disadvantages to post-fix the tissues for more than 24 hours (2 days-1
week) for further immunohistochemistry experiments? Thank you very much
in advance.
Sincerely Yours,
Salim Inan, PhD
HBI, University of Calgary
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
lei...@buffalo.edu
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