I agree with you Geoff Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Thursday, 23 September 2010 11:59 PM To: Salim Yalcin Inan Cc: Histonet Subject: Re: [Histonet] post-fixation question Merced is correct about longer fixation inducing more cross-linking, possibly making immunostaining more difficult. Much depends on the antigen you are looking for. I disagree that the tissue will become brittle, this is not my experience. Geoff Salim Yalcin Inan wrote: > Hello, > > > > I have a question about post-fixation. As far as I know, for most of > the immunohistochemistry studies in mouse/rat brain, post-fixation > period is between 2 hours to 24 hours in 4% paraformaldehyde at +4 > Celsius followed by cryoprotection with 20-30% sucrose solution. Are > there any disadvantages to post-fix the tissues for more than 24 hours > (2 days-1 week) for further immunohistochemistry experiments? Thank > you very much in advance. > > Sincerely Yours, > > Salim Inan, PhD > > HBI, University of Calgary > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet