You can test the activity of the Schiffs. Add a few drops of formalin to a small amount of Schiffs. There should be the typical pink stain. The pH should be about 2. If there are white flakes in the bottle, this could be a sign for a too high pH.
Is your periodic acid ok? Gudrun -----Ursprüngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Diana McCaig Gesendet: Mittwoch, 06. Oktober 2010 17:03 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] PAS STAIN Hi We have been doing a PAS stain on the same control block and same reagent supplier for a long time. Yesterday when we ran the slides, they failed to stain We opened new bottles of Periodic Acid and Schiff's Reagent and recut fresh controls Still, we are unable to get any staining. Suggestions to help would be appreciated Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet