I know there were a ton of posts about this, but I deleted them o= n accident...Did you test your Schiff's? Put a drop of 10% formalin i n it...if it turns pink, it's good...if not, throw it out =)
Sarah Goebel= , B.A., HT (ASCP) Histotechnician XBiotech US= A Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 A= ustin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: Re: [Histonet] PAS STAIN From: Geoff McAuliffe <[1]mcaul...@um= dnj.edu> Date: Wed, October 06, 2010 1:28 pm To: Diana McCaig <[2][email protected].= ca>, "Histonet ((E-mail))" <[3][email protected]= outhwestern.edu> I make my Schiff's from scratch and store it in the refrigerator. I use a minimum amount of charcoal and I heat it in the oven at over 100 C the night before to be sure it is nice and dry. I make periodic acid fresh that day. I make bisulfite rinses fresh that day. Aqueous formalin for 48 hours at room temp. is OK for rat and mouse liver glycogen, I don't know about other species. Slices of liver should be thin. When in doubt formalin+alcohol+acetic acid is an excellent fixative. Geoff Diana McCaig wrote: > Hi > We have been doing a PAS stain on the same control block and same > reagent supplier for a long time. > Yesterday when we ran the slides, they failed to stain > > We opened new bottles of Periodic Acid and Schiff's Reagent and recut<= br> > fresh controls > > Still, we are unable to get any staining. > > Suggestions to help would be appreciated > > Diana > _______________________________________________ > Histonet mailing list > [4][email protected]= southwestern.edu > [5]= http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 [6][email protected] ********************************************** _______________________________________________ Histonet mailing list [7][email protected]= western.edu [8]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:[email protected]"; 2. 3D"mailto:[email protected]"; 3. 3D"mailto:[email protected]"; 4. 3D"mailto:[email protected]"; 5. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"; 6. 3D"mailto:[email protected]"; 7. 3D"mailto:[email protected]"; 8. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"; _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
