Donna, I would also check to make sure that the tissue wasn't held in an something with a lot of alcohol, like RNA later. I found out first hand that it doesn't work well. Good luck
On Thu, Feb 10, 2011 at 11:38 AM, Reynolds,Donna M <dreyn...@mdanderson.org> wrote: > Has anyone ever experienced OCT blocks that cut like they are soft. I have > turned the cyrostat down 10 degrees lower than usual and they are still soft. > Really weird part is that I have 20 blocks in this group and some are soft > and others are just fine. Tried changing angle, new blade nothing helps. When > I finally manage to get a decent section and pick it up on the slide, the OCT > around the tissue want even pick up smooth it wrinkles really bad. > I tried just a block of OCT that I froze on a chuck and it cut great. I am > clueless as to what is going on. > Samples were frozen by someone else and brought to me to be cut. > Donna Reynolds Core IHC Lab > M.D. Anderson Cancer Center, Houston TX > Research lab > 713-192-8106. > > > Donna Reynolds Core IHC Lab > Dept. Cancer Biology, SRB 1.660 > 713-792-8106 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
