I agree with previous responders to this query, as I've gotten alcohol on
my blocks and since alcohol doesn't freeze real well it makes a sticky mess
with the OCT. The affected OCT can be scraped off with a razor blade.
Hopefully the tissue itself did not come in contact with alcohol prior to
embedding.
Regards,
Merced
--On Thursday, February 10, 2011 12:51 PM -0800 Patrick Laurie
<foreig...@gmail.com> wrote:
Donna,
I would also check to make sure that the tissue wasn't held in an
something with a lot of alcohol, like RNA later. I found out first
hand that it doesn't work well. Good luck
On Thu, Feb 10, 2011 at 11:38 AM, Reynolds,Donna M
<dreyn...@mdanderson.org> wrote:
Has anyone ever experienced OCT blocks that cut like they are soft. I
have turned the cyrostat down 10 degrees lower than usual and they are
still soft. Really weird part is that I have 20 blocks in this group and
some are soft and others are just fine. Tried changing angle, new blade
nothing helps. When I finally manage to get a decent section and pick it
up on the slide, the OCT around the tissue want even pick up smooth it
wrinkles really bad. I tried just a block of OCT that I froze on a chuck
and it cut great. I am clueless as to what is going on. Samples were
frozen by someone else and brought to me to be cut. Donna Reynolds Core
IHC Lab
M.D. Anderson Cancer Center, Houston TX
Research lab
713-192-8106.
Donna Reynolds Core IHC Lab
Dept. Cancer Biology, SRB 1.660
713-792-8106
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--
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plau...@cellnetix.com
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)
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