Christopher I use Americlear and have not had a staining problem. Your last alcohols must be absolute and as stated previously your mounting media must be compatible. I do not, however, use Americlear on the processor as we did have processing problems there. We use ProPar from Anatech on the processor. With the expense and carcinogen factor of Xylene, this set up works great for us. As with any clearing agent, water contamination , produces leching eosin as one of many problems.
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Christopher Conlisk <cconl...@kcskincenter.com> Sent by: histonet-boun...@lists.utsouthwestern.edu 06/03/2011 02:17 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Slide Bright Question Hello Everyone, I have worked in labs that use Xylene my entire career and I just started at a Lab That only uses a Xylene Substitute "Slide-Bright". I am having problems with the H&E. After staining and coverslipping (The slides look fine innitially), Then about 5-10 minutes after coverslipping the Eosin starts bleeding out all around the tissue. I have asked several of my Histotech Friends that are old timers and they say that Xylene Substitutes are awful at deparrifinization and awful at clearing. They told me that the alcohol isnt getting thoroughly cleared in the "Slide Brite" and then it is eventually leeching out after coverslipping??? Is this true and does anyone have any guidance for this issue? We also run MOHS slides on the same stainer and I keep all the reagents clean as a whistle. I really hate Xylene Substitute's.... Thanks C.S. Conlisk HT(ASCP), PBT(ASCP) Kansas City Skin and Cancer Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet