Hello all, Has anyone used the reagent 'Uni-trieve' from Innovex? It is purported to be a universal antigen retrieval solution that can be used at lower temperatures (65-70 C for cytoplasmic antigens, and 75-80 C for nuclear antigens):
http://innvx.com/unitrievepage.html The company claims that the reagent is a universal retrieval solution for all antibodies and tissues (which is silly of course - how could they know?). Does anyone have any experience with the product? I am interested because I see that increased heat during antigen retrieval causes greater tissue autofluorescence during immunofluorescence stainings. My current protocol is to use 20 minutes at 90-95 C on a hot plate using sodium citrate buffer. Does anyone else either Uni-trieve or a different reagent/protocol for immunofluorescence stainings? Thanks, Andrea Andrea Marion Graduate Student University of Illinois at Chicago _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
