Hi Sarah, Thanks for the info! Have you used Unitrieve for immunofluorescence? I am wondering whether I will see a reduction in the autofluorescence caused by heating? I will request a sample to try and report back to all.
Andrea Andrea Marion Graduate Student University of Illinois at Chicago On Tue, September 20, 2011 12:49 pm, sdys...@mirnarx.com wrote: > I actually attended a class they put on a few months ago where they used > Unitrieve. It works really well. It doesn't seem to produce any kind > of background and yes, it is used at much lower temps. However the > retrieval process takes a little longer, but...you can put it in an even > lower temp. water bath overnight, come in to work in the morning, and > your slides are reading to go. > Contact Zara over there and she might be able to hook you up with a > sample. > Good Luck!! > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrea > Marion > Sent: Tuesday, September 20, 2011 11:59 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence > > Hello all, > > Has anyone used the reagent 'Uni-trieve' from Innovex? It is purported > to > be a universal antigen retrieval solution that can be used at lower > temperatures (65-70 C for cytoplasmic antigens, and 75-80 C for nuclear > antigens): > > http://innvx.com/unitrievepage.html > > The company claims that the reagent is a universal retrieval solution > for > all antibodies and tissues (which is silly of course - how could they > know?). Does anyone have any experience with the product? > > I am interested because I see that increased heat during antigen > retrieval > causes greater tissue autofluorescence during immunofluorescence > stainings. My current protocol is to use 20 minutes at 90-95 C on a hot > plate using sodium citrate buffer. Does anyone else either Uni-trieve or > a > different reagent/protocol for immunofluorescence stainings? > > Thanks, > > Andrea > > Andrea Marion > Graduate Student > University of Illinois at Chicago > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet