That decision needs to be made by your Medical Director. In my laboratory we use polymer detection for almost all of our IHCs and, therefore, I don't require a "Negative Reagent Control" for those cases. We have one antibody that requires avidin-biotin detection and we run a negative reagent control in parallel whenever we run that antibody. In my experience, if you are using polymer detection and your antibodies are properly optimized and validated, the negative reagent control slide serves no useful purpose and, therefore, is not needed.
Best wishes to everyone on Histonet for the coming New Year! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> <jsjurc...@comcast.net> 12/28/2012 3:18 PM >>> How is everyone interpreting the CAP rule about using negative controls? Do we still need a negative with each patient slide? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
