Hi Colleen, I would say it's unusual, but not completely impossible that EDTA has interfered with your IHC. We had that problem with demonstrating B-galactosidase in mouse bones. If we decalcified it in EDTA after whole mount staining in X-gal, the blue staining was removed. But if we decalcified it in formic acid, the stain was retained.
I think a few years ago, Biogenex had a room temperature antigen retrieval solution for acid decalcified bone (not the same as your situation, I know). But I think it was largely an alkaline solution you let the slides sit for maybe 30 minutes prior to staining. Might be worthwhile to try a different retrieval method for these and see what happens. Otherwise, are you sure you are using a clone that reacts in mouse tissue? We use Rabbit monoclonal SP6. Teri Johnson Manager, Histology GNF - San Diego, CA 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
