We experienced different IHC staining qualities on bone marrow, that was decalcified with EDTA or formic acid. I remember, our CD38 antibody was always very weak after EDTA and made no problems after formic acid. I think it is very difficult to make an over-all-statement for all antigens/all antibodies. Gudrun
-----Ursprüngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Teri Johnson Gesendet: Freitag, 16. August 2013 19:54 An: 'cfors...@umn.edu' Cc: histonet@lists.utsouthwestern.edu Betreff: [Histonet] EDTA decal Hi Colleen, I would say it's unusual, but not completely impossible that EDTA has interfered with your IHC. We had that problem with demonstrating B-galactosidase in mouse bones. If we decalcified it in EDTA after whole mount staining in X-gal, the blue staining was removed. But if we decalcified it in formic acid, the stain was retained. I think a few years ago, Biogenex had a room temperature antigen retrieval solution for acid decalcified bone (not the same as your situation, I know). But I think it was largely an alkaline solution you let the slides sit for maybe 30 minutes prior to staining. Might be worthwhile to try a different retrieval method for these and see what happens. Otherwise, are you sure you are using a clone that reacts in mouse tissue? We use Rabbit monoclonal SP6. Teri Johnson Manager, Histology GNF - San Diego, CA 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet