Good idea. I have to soak the edta decaled tissues in an alkaline solution inorder to restore enzyme histochemical staining for TRAP, might be the same issue for some IHC.
Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com rueggihcconsultin...@outlook.com This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Friday, August 16, 2013 11:54 AM To: 'cfors...@umn.edu' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] EDTA decal Hi Colleen, I would say it's unusual, but not completely impossible that EDTA has interfered with your IHC. We had that problem with demonstrating B-galactosidase in mouse bones. If we decalcified it in EDTA after whole mount staining in X-gal, the blue staining was removed. But if we decalcified it in formic acid, the stain was retained. I think a few years ago, Biogenex had a room temperature antigen retrieval solution for acid decalcified bone (not the same as your situation, I know). But I think it was largely an alkaline solution you let the slides sit for maybe 30 minutes prior to staining. Might be worthwhile to try a different retrieval method for these and see what happens. Otherwise, are you sure you are using a clone that reacts in mouse tissue? We use Rabbit monoclonal SP6. Teri Johnson Manager, Histology GNF - San Diego, CA 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet