It's a bad question. Generally the "best" temperature is probably between 55 and 65, depending on the paraffin. This would fall between the two latter answers. Hmm.... What to do?
Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_man...@merck.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Monday, September 09, 2013 9:28 AM To: histonet Cc: Naujokas, Agne; Meier, Melissa Subject: [Histonet] (no subject) Good morning all! One of our fellows emailed me a question that she came across while studying for her boards: "I'm studying for my board exam and came across questions re: paraffin embedding. It reads: best temperature for paraffin embedding is 38-48 48-58 58-70. I am getting some info on Internet that says 58 but is the range lower or higher than that? What do we use?" This seems to me to be an odd question because it depends on the melting point of the paraffin in use. Ours melts at 58C and we embed at 60C, but we have also used paraffin that melts at 56C and we embedded at 58C. Or am I missing something? Does anyone have a clear cut answer to this? Thanks everyone! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
