The last answer will cover most paraffins used routinely and may cover microwave also. Temperatures below 58C are often melt points however; they may not be a complete liquid or have slightly increased viscosity.
Pam Marcum UAMS -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Monday, September 09, 2013 8:28 AM To: histonet Cc: Naujokas, Agne; Meier, Melissa Subject: [Histonet] (no subject) Good morning all! One of our fellows emailed me a question that she came across while studying for her boards: "I'm studying for my board exam and came across questions re: paraffin embedding. It reads: best temperature for paraffin embedding is 38-48 48-58 58-70. I am getting some info on Internet that says 58 but is the range lower or higher than that? What do we use?" This seems to me to be an odd question because it depends on the melting point of the paraffin in use. Ours melts at 58C and we embed at 60C, but we have also used paraffin that melts at 56C and we embedded at 58C. Or am I missing something? Does anyone have a clear cut answer to this? Thanks everyone! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
