I routinely cut 50 or even 200um sections. The brain should have some variations of light beige to it. If it is too white, it is too cold. What are you using? I have found that a microtome works much better for thicker slices than a cryostat.
Good luck! Laura -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kruttwig, Klaus Sent: Wednesday, October 23, 2013 3:22 PM To: Grantham, Andrea L - (algranth); HISTONET Subject: [Histonet] RE: cutting thick brain frozen sections for Golgi stain Hi Andrea, did you try it with a vibratome? If not, I would recommend it. Thanks, Klaus ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Grantham, Andrea L - (algranth) [algra...@email.arizona.edu] Sent: Wednesday, October 23, 2013 3:01 PM To: HISTONET Subject: [Histonet] cutting thick brain frozen sections for Golgi stain I need to cut 80 micron sections of frozen brain. Tried today at -18 degrees C but the brains kept cracking. I read that the temp should be -3 to -13 degrees C. Has anybody cut sections this thick of brain? Can you pass along any words of inspiration? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu<mailto:algra...@email.arizona.edu> Tel: 520.626.4415 Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
