http://news.discovery.com/human/health/why-ebola-wont-spread-in-the-united-states-141009.htm

http://www.voanews.com/media/video/us-doctors-learn-from-treating-ebola-patients/2477893.html

http://www.washingtonpost.com/lifestyle/travel/tips-for-traveling-to-africa-in-the-age-of-ebola/2014/10/09/46af8ae8-4efd-11e4-8c24-487e92bc997b_story.html



In our Emory news today... I haven't listened yet, but you all might like to 
look...

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph’s 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.


-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson
Sent: Thursday, October 09, 2014 5:32 PM
To: 'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu); 
'Finley, Sue [PH]'
Subject: RE: [Histonet] Ebola

Correction,  due to years of formalin abuse,  it was 1 hour in concentrated 
formic acid.  We then processed and cut them with all other cases.  A few cases 
slipped through with just formalin fixation and additional precautions needed 
to be taken.  That said the easiest  and primary route of infection for CJD is 
ingestion.  Needless to say we did not eat any of the tissue.


A simple and effective method for inactivating virus infectivity in 
formalin‐fixed tissue samples from patients with Creutzfeldt‐Jakob disease

Paul Brown, MD,
 Axel Wolff, DVM and
 D. Carleton Gajdusek, MD

-Show Affiliations

Laboratory of CNS Studies, National Institute of Neurological Disorders and 
Stroke, National Institutes of Health, Bethesda, MD.



doi: 10.1212/WNL.40.6.887  Neurology June 1990   vol. 40  no. 6  887



Abstract
Full Text (PDF)


Abstract

We fixed brains from hamsters infected with scrapie virus in (1) formalin, (2) 
phenol-saturated formalin, (3) formalin with a 1-hour immersion in formic acid, 
or (4) phenol-saturated formalin with a 1-hour immersion in formic acid. In 
addition, we used the formalin-formic acid procedure on brains from mice 
infected with the virus of Creutzfeldt-Jakob disease. Formic acid proved 
superior to phenol in respect to both disinfection and tissue preservation, 
almost completely eliminating virus infectivity in sections that were 
histologically indistinguishable from formalin-fixed material. The inclusion of 
a formic acid step in routine formaldehyde tissue fixation will thus provide 
histologic sections of excellent quality, and virtually eliminate the risk of 
handling infectious material in the subsequent neuropathologic processing of 
tissues from patients with CJD.
© 1990 by the American Academy of Neurology


James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation Scientific 
Technical Leader II, Histology Tel    858-332-4647 Fax   858-812-1915 
jwat...@gnf.org


-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson
Sent: Thursday, October 09, 2014 1:57 PM
To: 'Emily Brown'
Cc: Histonet@Lists. Edu
Subject: RE: [Histonet] Ebola

Back in the 80 NIH did extensive studies on CJD,  their protocol requires 24 
hours in formalin, 24 hours in formic acid, followed by 48 hours in formalin, 
then paraffin processing.  Worked with CJD at Frederick Cancer Research in 
collaboration with the group from NIH.  If I find the publications I will 
forward them.

James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation Scientific 
Technical Leader II, Histology Tel    858-332-4647 Fax   858-812-1915 
jwat...@gnf.org


-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Brown
Sent: Thursday, October 09, 2014 1:40 PM
Cc: Histonet@Lists. Edu
Subject: Re: [Histonet] Ebola

If formalin didn't kill CJD, what did you use? Just curious.

Emily

"By bitching and bitching and bitching, they could exhaust the drama of their 
own horror stories. Grow bored. Only then could they accept a new story for 
their lives. Move forward."

-Chuck Palahniuk, "Haunted"

On Thu, Oct 9, 2014 at 4:34 PM, Patsy Ruegg <prueg...@hotmail.com> wrote:

> Well said Pam, it is just assumed that formalin will eliminate the
> biohaz for Ebola, I doubt if that has been conclusively proven yet,
> remember we only discovered fairly recently that formalin fixation did
> not protect us from CJD!!!!
>
> Patsy Ruegg, HT(ASCP)QIHC
> Ruegg IHC Consulting
> 40864 E Arkansas Ave
> Bennett, CO 80102
> H 303-644-4538
> C 720-281-5406
> prueg...@hotmail.com
> pru...@ihctech.net
>
>
> > Date: Thu, 9 Oct 2014 20:26:16 +0000
> > From: mucra...@comcast.net
> > To: tanyaabb...@catholichealth.net
> > Subject: Re: [Histonet] Ebola
> > CC: histonet@lists.utsouthwestern.edu
> >
> > Take Brett's advise and use that as guidleine.  We don't know as
> > much as
> we should about these viruses.   Pam
> >
> > ----- Original Message -----
> >
> > From: "Tanya Abbott" <tanyaabb...@catholichealth.net>
> > To: "Histonet" <histonet@lists.utsouthwestern.edu>
> > Sent: Thursday, October 9, 2014 2:03:47 PM
> > Subject: [Histonet] Ebola
> >
> > Dare I ask?! Are any Pathology labs discussing what to do with
> specimens/precautions, etc. regarding a person with a potential Ebola
> infection?
> >
> > Tanya G. Abbott RT (CSMLS)
> > Manager Technologist, Histology/Cytology St. Joseph Medical Center
> > Reading, PA 19603-0316 ph  610-378-2635 fax 610-898-5871
> > email: tanyaabb...@catholichealth.net
> >
> > This electronic mail and any attached documents are intended solely
> > for
> the named addressee(s) and contain confidential information. If you
> are not an addressee, or responsible for delivering this email to an
> addressee, you have received this email in error and are notified that
> reading, copying, or disclosing this email is prohibited. If you
> received this email in error, immediately reply to the sender and
> delete the message completely from your computer system.
> > _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

________________________________

This e-mail message (including any attachments) is for the sole use of
the intended recipient(s) and may contain confidential and privileged
information. If the reader of this message is not the intended
recipient, you are hereby notified that any dissemination, distribution
or copying of this message (including any attachments) is strictly
prohibited.

If you have received this message in error, please contact
the sender by reply e-mail message and destroy all copies of the
original message (including attachments).
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Reply via email to