Depending on the reaction of the glomerulii to the Dengue virus and the stage
of such reaction perhaps a PAM (periodic acid methenamine silver) stain could
show something.René J.
On Wednesday, October 22, 2014 11:31 AM, "Rodriguez, Kristofer"
<kristofer.rodrig...@bcm.edu> wrote:
One of my clients has been performing experiments with a mouse model of Dengue
Virus Infection. They have been treating the mice with monoclonal antibodies
(client did not specify what antibody)as a potential treatment for dengue virus
infection. They have harvested the kidneys and put them in 10% NBF. They want
to know if routine H&E will show any antibody deposits.
I told him that I would not be able to directly observe the antibody deposit
itself, however I might be able to observe the reaction to the antibody deposit
in the basement membrane of the glomeruli as a membranous glomerulopathy on an
H&E.
I was wondering if I should do any special stains that might show more than an
H&E.
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