Hi,
may be you can try to label murine immunoglobulins. A mere secondary
against murine IgGs could do the trick, depending on which isotype
your client is suspecting to form the precipitates.
Just an idea, never have tried before.
Cheers
Julio
"Rodriguez, Kristofer" <kristofer.rodrig...@bcm.edu> escribió:
One of my clients has been performing experiments with a mouse model
of Dengue Virus Infection. They have been treating the mice with
monoclonal antibodies (client did not specify what antibody)as a
potential treatment for dengue virus infection. They have harvested
the kidneys and put them in 10% NBF. They want to know if routine
H&E will show any antibody deposits.
I told him that I would not be able to directly observe the antibody
deposit itself, however I might be able to observe the reaction to
the antibody deposit in the basement membrane of the glomeruli as a
membranous glomerulopathy on an H&E.
I was wondering if I should do any special stains that might show
more than an H&E.
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