Hi, I think this is comparable with IF for detecting human autoimmun antibodies in skin or kidney.
The human immunglobulins are detected with an fluorochrome conjugated antibody mouse-anti-human IgG (for example). In your case it may be successful to stain with a goat-anti-xxx IgG / IgM /IgA etc. On human FFPE kidney we performed pretreatment with pepsin (3-5 min) and then 30 min polyclonal anti-serum 1:10 and 1:20. To show immunglobulin-deposits in the glomeruli you can perform a trichrome-stain like MSB or SFOG. Protein deposits will be shown bright red, but are not distinguishable in the kind of protein. It will depend on the amount of deposit, I assume. Hope this helps Gudrun -----Ursprüngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Rodriguez, Kristofer Gesendet: Mittwoch, 22. Oktober 2014 17:31 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Antibody deposit visualization One of my clients has been performing experiments with a mouse model of Dengue Virus Infection. They have been treating the mice with monoclonal antibodies (client did not specify what antibody)as a potential treatment for dengue virus infection. They have harvested the kidneys and put them in 10% NBF. They want to know if routine H&E will show any antibody deposits. I told him that I would not be able to directly observe the antibody deposit itself, however I might be able to observe the reaction to the antibody deposit in the basement membrane of the glomeruli as a membranous glomerulopathy on an H&E. I was wondering if I should do any special stains that might show more than an H&E. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet