Sometimes these issues are "mysterious" and in your case are particularly
"mysterious" because you once solved the problem, only to recently "reappear".I
am going to tell you how I used to do it and never had those PT rejections:1-
for both hematoxylin and eosin I kept a log of the number of stained slides and
changes both when I reached 200, regardless of the day of the week or the time
within the day the amount was reached.2- I always used the same brand (Harleco)
3- I never left the staining set over the week. Everything was removed the last
day of work (Saturday) and the containers were rinsed and dried and left upside
down. For the automatic stainer (Sakura) we did the same thing.4- come Monday
morning the whole set was prepared and everything started again.
Now, before I retired all the method was changed and this part probably you
will not attempt: we dewaxed with dishwasher soap (elimination of xylene and
ethanol). The slides went directly to the hematoxylin → bluing →
differentiation → water → eosin → wash water and from then directly to an oven
at 60ºC for 15 minutes (elimination of ethanol and xylene) → coverslip
So, try as I describe in points 1 to 4 or you can even could try eliminating
xylene and ethanol and "go green" and "dry".René
On Monday, July 13, 2015 1:30 PM, "Hannen, Valerie"
<valerie.han...@parrishmed.com> wrote:
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{}#yiv6464311563 Rene, As I stated before and maybe I was not entirely clear,
we had this problem ( little Eosin in the sections of the first rack of slides
on Monday morning) before, but had remedied it by starting to change the Eosin
on Monday morning before the first rack of slides was run. He now is stating
that the problem of little Eosin in the sections from the first rack on Monday
A.M. is starting again. Since the last fix of the problem we have not deviated
in any way, procedure or chemical wise. We have used the same lot # in
previous weeks for the Eosin as we currently have on the stainer ( I changed
the Eosin this morning) , so I don’t think it is an Eosin problem per se.
Kari, We do use 95% alcohol before our Eosin at all times. I would think that
if the alcohol before the Eosin was the problem, all of my racks would have
the “little Eosin in the sections” problem.. not just the first rack. Again
any and all suggestions are welcomed!! Thanks again Rene and Kari for
replying. Valerie From: Rene J Buesa [mailto:rjbu...@yahoo.com]
Sent: Monday, July 13, 2015 11:20 AM
To: Hannen, Valerie; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Eosin It is difficult to advise you anything without
knowing what is your PT's complaint. What he does complain about?
René On Monday, July 13, 2015 11:17 AM, "Hannen, Valerie"
<valerie.han...@parrishmed.com> wrote: Good morning,
I once again am dealing with a my picky Pathologist!! About a year ago, he
started to complain about not having enough Eosin on his sections( first
morning rack on Monday).. I went from changing the Eosin on Friday and stirring
it on Monday to totally changing it on Monday...it has all been good until the
end of last week. The problem has started up again... little Eosin in the first
rack of Monday morning. Any suggestions??
Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com<mailto:valerie.han...@parrishmed.com>
www.parrishmed.com
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