Hi Elizabeth, We are an aquaculture histology laboratory and routinely use four parts 1% Eosin Y (aq) to one part Putts eosin for the same reason that you describe. 1% aqueous or alcoholic alone just doesn't do the job.
Debbie Faichney, BSc Senior Technician Histology/Bacteriology Laboratories Institute of Aquaculture University of Stirling Stirling, FK9 4LA Scotland UK -----Original Message----- From: Cameron, Elizabeth via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: 30 August 2016 15:25 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Hi, I have been staining fish tissues fixed in Davidsons with H&E, and the researcher would like the eosin to be more intense. Our standard protocol works well for our own tissue, but the fish look much more washed out. I am using alcoholic eosin Y, have tried both water and alcohol before and I have varied the alcohol differentiation steps after the Eosin. I also extended the time in Eosin and increased the wash after bluing to make sure the sections are not basic. Any suggestions would be appreciated. Thank you. Elizabeth M. Cameron, HT(ASCP), QIHCCM Lead Histologist Mid Coast Hospital 123 Medical Center Drive Brunswick, ME 04011 (207) 373-6573 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The University achieved an overall 5 stars in the QS World University Rankings 2015 The University of Stirling is a charity registered in Scotland, number SC 011159. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
