You might try some combination of eosin and biebrich scarlet and/or
phloxine.
Adding a little acetic acid might help.
http://www.stainsfile.info/StainsFile/downloads/eosin.pdf
I am quite fond of Biebrich Scarlet and like way it stains brain and
heart particularly.
Biebrich is much more red than eosin.
Phloxine is an aggressive pink.
*
A bit punny about the fish looking washed out.
*
"There was something fishy about the butler.
Probably a Pisces working for scale." - Phil Proctor
On 08/30/2016 10:25 PM, Cameron, Elizabeth wrote:
Hi,
I have been staining fish tissues fixed in Davidsons with H&E, and the
researcher would like the eosin to be more intense. Our standard protocol works
well for our own tissue, but the fish look much more washed out. I am using
alcoholic eosin Y, have tried both water and alcohol before and I have varied the
alcohol differentiation steps after the Eosin. I also extended the time in Eosin
and increased the wash after bluing to make sure the sections are not basic. Any
suggestions would be appreciated.
Thank you.
Elizabeth M. Cameron, HT(ASCP), QIHCCM
Lead Histologist
Mid Coast Hospital
123 Medical Center Drive
Brunswick, ME 04011
(207) 373-6573
--
E. Wayne Johnson 朱稳森博士
Enable AgTech Consulting
恩睿康农业技术咨询有限公司
Beijing
188 1088 3205
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